LIKE WOOH TECHNO VOL 2
Download ->>->>->> https://urllie.com/2tCFRo
STRONG BAD: {typing} Whoa! You must go to a pretty cool school. Most schools just have a marching band or maybe a jazz ensemble. But a death metal band That's extracurriculariffic! {clears screen} Now let's see, first, you'd better be ugly, Dane. Or fat. The gift of death metal does not smile on the good looking. If there's any doubt, go ahead and hot-glue some corn flakes to your face. That'll do the trick. Now, I'm no geographist, but I don't think Oregon is anywhere near Scandinavia, which seems to be the only place left where death metal will get you any chicks. {clears screen} As for lyrics, you've got it all wrong! Screaming words at the top of your lungs is for people with blond hair. Ya know, words like 'tonite,' 'wooh-mon,' and the name of any street in L.A. {types \"run 'webcam'\"} Check it out! {clears screen. A window with a camera shot of Larry Palaroncini pops up.}
LARRY PALARONCINI: {sings the following lines, which appear below the shot under the screen name \"ladeezluvlarry71\"} tonight! wooh-mon! we'll be drivin' down Sepulveda with the t-top down
STRONG BAD: {typing} So for death metal, you want to scream from the bowels of your lungs. The bowels! Words like, {in a deep, sinister voice, and with the words in green, spooky letters} 'decay,' 'deranged,' 'decrepit,' and um, 'delouse.' In fact, you really can't go wrong with anything that starts with d-e. 'Cept for maybe
STRONG BAD: Creeping... rusty... meat. Truly the heart and soul of all death metal. Except, now I feel like I really need to brush my teeth. Lookin' at all that rust. And beef. While I'm gone, complete this worksheet I've prepared for you aspiring death metal types. And, uh, careful with the hot-glue gun, Dane.
STRONG BAD: He's too happy. Yeah, clearly that's the only guy they could find. He's just like, \"Well, I don't really listen to the music you guys play, but I'll do it! We're gonna be in the battle of the bands\"
so i dunno if its gone forever but the artist is Jason Revele or something very similar to that last name and the song was titled Just Remember , the lyrics start with {i am simply am not there} and say { with chris and layal too }maybe like 5 or 6 years ago hes a local white rapper talks about the father/ creator hes also from BC AND super lowkey might of been charged for sexual assault on a minor lol please and in the video hes got footage of him on stage doing the song help please i hate google search
Anyone remember a music video from late 1970s or 1980 (no later). I watched it on TV in Netherlands. Sung in English. Same vibe as Taco Puttin on the Ritz, but not that song. Had a Big Band with mannequins playing trombone, as I recall. Would like to watch it again and get it out of my head.
In addition to the ordinary albums in the series, MuchMusic also released albums such as MuchDance 80's (2005) that included classics like Paula Abdul's \"Straight Up.\" There has also been a volume of MuchDance 90s released, along with two #1 hits compilations.
Vaccination against ND is commonly practised around the world and plays a critical role in disease control. Vaccination offers protection against clinical disease and morbidity and can reduce the amount of virus shed upon exposure to a field isolate, consequently limiting the spread to new susceptible hosts. As lentogenic (and asymptomatic enteric) viral strains cause asymptomatic infection in SPF chickens, they are commonly used to develop attenuated-live and inactivated egg-produced ND vaccines for commercial poultry (Collett and Smith, 2020). Live vaccines are suitable for mass application and elicit both cellular and humoral responses, although the immunity can be short-lived (Dimitrov et al., 2017; Collett and Smith, 2020; Nurzijah et al., 2022; World Organization for Animal Health (WOAH), 2022). Inactivated vaccines are considered to be safer than live vaccines and induce good humoral responses but require direct immunization (intramuscularly or subcutaneously), as well as formulation with an adjuvant and/or multiple vaccine doses to enhance the immunogenicity. Egg-based vaccine production is a well-established and cost-effective technology, although both live and inactivated vaccine production involve the handling of infectious viral material, which poses biosafety concerns and necessitates biocontainment facilities. Furthermore, due to the genetic variation between the F and/or HN sequences of lentogenic vaccine strains and contemporary ND isolates, the capacity of these live and inactivated vaccines to confer protection and effectively reduce viral shedding in vaccinated birds is likely reduced (Miller et al., 2013; Dimitrov et al., 2017). Recently, recombinant viral vector vaccines expressing the ND HN and/or F proteins have been approved for use. Recombinant viral vector vaccines effectively stimulate both humoral and cellular immune responses, like live vaccines, but with superior safety, a shorter production time, and Differentiating Infected from Vaccinated Animals (DIVA)-potential. The production of these recombinant viral vector vaccines using the established low-cost egg-based production platform has been extensively investigated, although their production in cell culture systems is also possible, albeit at a higher production cost. For poultry vaccines, low manufacturing costs are imperative to ensure production margins are maintained (Dimitrov et al., 2017; Fulber and Kamen, 2022; Nurzijah et al., 2022; World Organization for Animal Health (WOAH), 2022).
The capacity to differentiate between infected and vaccinated animals (DIVA) is of great importance for disease surveillance, diagnostic and/or export purposes (ND-free status). Due to the absence of internal viral proteins, VLP vaccines have DIVA-potential with the use of a combination of appropriate serological tests (Liu et al., 2013). In the present study, ND HN- and F-specific antibodies were detected using routine tests (HIs and an anti-NDV F commercial ELISAs, respectively) following vaccination with the plant-produced NDV F/HN VLPs. By making use of another commercial ELISA kit that targets antibodies against an internal ND protein, for example the ID Screen ELISA (ID.Vet, France) that detects nucleoprotein (NP)-specific antibodies, DIVA could be attained by confirming the absence of NP-specific antibodies in the sera that tested positive for F- and/or HN-specific antibodies. In practice however, antigen-matched VLP vaccines are likely to be applied in combination with live vaccines in the field, for maximum protection.
Transient plant-based expression is a cost-effective and scalable production platform. Based on the yields obtained here, conservatively estimated, one kilogram of infiltrated leaf material would be sufficient for the prime-boost vaccination of 10,000 chickens (1024 HA units (10 log2) per dose). In the field, however, the ND VLP vaccine would likely be applied as a booster to a live vectored vaccine given in the hatchery, which would mean that up to 20,000 chickens could receive a booster from one kilogram of infiltrated leaf material. A high antigenic dose of 1024 HAU was used in the present immunogenicity study to ensure measurable antibody titres were elicited following vaccination with the ND VLPs, but the prime-boost vaccination of insect cell-produced ND VLPs with an HA titre of 256 HAU (8 log2) was previously efficacious in chickens against viral challenge, resulting in an increased protection period, a reduction in viral load, and a shortened shedding period in comparison to a commercial live La Sota ND vaccine (Xu et al., 2019). At the latter dose, the potential number of chickens vaccinated per kilogram of infiltrated leaf material could increase up to 40,000. Furthermore, according to Ruiz et al. (2018), minimally processed VLPs could potentially be used as immunogen and would especially be beneficial for veterinary vaccines due to the cost implication of the reduction in downstream processes. Plant-produced F/HN VLPs that were only subjected to clarification yielded an HA titre of 1:512 (9 log2) i.e., double the effective ND VLP antigenic dose reported by Xu et al. (2019), therefore, a future aim is to investigate the toxicity an immunogenicity of minimally processed plant-produced VLPs in chickens. There is also potential for mass application of VLP vaccines via in ovo (Schädler et al., 2019) and intranasal administration (Earnest, 2009) with suitable adjuvants, which would be an enormous advantage for commercial poultry production. 781b155fdc